Comparison of Rapid Freezing and Vitrification for Human Sperm Cryopreservation Using Trehalose as a Cryoprotective Agent
نویسندگان
چکیده
منابع مشابه
Cryopreservation of Endothelial Cells in Various Cryoprotective Agents and Media – Vitrification versus Slow Freezing Methods
Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled-rate -1°C/minute cell freezing in a -80°C freezer and tested four cryoprotective agents (CPA), namely dimethyl sulfoxide (DMSO), ethylene glycol (...
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Background: The importance of mice as animal model for research has promoted the surge of many strains with important characteristics which need to be preserved. Embryo cryopreservation appears as the most suitable technique. However, until now there is not an effective methodology for this specie. This study aimed to evaluate three methods for mice embryos cryopreservation. Materials, Methods ...
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Background: The aim of this study was to evaluate the efficacy of a vitrification protocol developed by our group to cryopreserve ovarian tissue. For this, we used ovaries from non-human primates in order to have an animal model close to the clinical setting. Materials and Methods: Ovarian biopsies from five adult baboons were vitrified, warmed and autografted. After five months, follicle survi...
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OBJECTIVE To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy. DESIGN Prospective randomized. SETTING Academically affiliated, private fertility center. PATIENT(S) Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were ran...
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ژورنال
عنوان ژورنال: Open Journal of Obstetrics and Gynecology
سال: 2019
ISSN: 2160-8792,2160-8806
DOI: 10.4236/ojog.2019.910136